Identification of Campylobacter species isolates with phenotypic methods and polymerase chain reaction.

INTRODUCTION Campylobacter jejuni (C. jejuni) and Campylobacter coli (C. coli) are the most common bacterial causes of enterocolitis in humans. However, identification of the species level is not always possible using standard biochemical tests. OBJECTIVE Therefore, the goal of this study was to identify these microorganisms by both phenotyping and polymerase chain reaction (PCR) technique. METHODS A total of 153 species of thermophilic campylobacters were examined with standard biochemical tests and PCR technique to prove hipO genes of C. jejuni and asp genes of C. coli. RESULTS Standard biochemical tests enabled the speciation of 121 strains of C. jejuni, while application of PCR detected 126 C. jejuni strains. CONCLUSION PCR technique allowed not only identification of hippurate-positive C. jejuni, but also hipurat-negative strains of C. jejuni which otherwise would be detected as C. coli if only biochemical tests were applied.